电离辐射通过转化生长因子-β-介导的腺体-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨伽马射线或是否可通过产物生长因子-β（TGF-β）-内源性的黏膜-糖受体转换 (EMT)来促进免疫系统的波及迁到。使用总量2Gy(60)Coγ线或反射由来人类器官的6种免疫系统，记录与EMT相关的变化，这包含分别借助显微镜技术，受体质印迹方法，免疫荧光技术，划痕试验和Transwell汤氏试验来观察并扫描细胞组织型态，EMT标记，波及迁到意志力等。运用于酶六轮免疫吸附法扫描这些免疫系统中TGF-β受体低水平，借助特别减缓剂SB431542来评估TGF-β路径通路在伽马射线或EMT中的作用。经过总量为2Gy反射的免疫系统中存在间叶细胞的表达，与假反射组相比其黏膜标记减少，间叶细胞标记提高，同时其波及转移意志力增强，TGF-β受体低水平也提高。进一步辨认出由A549伽马射线或诱发的EMT可通过对TGF-β路径减缓发生逆转。这些得出结论TGF-β内源性的EMT在伽马射线或诱发增强免疫系统波及转移意志力中起着关键作用。

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